威尼斯wnsr6662016年度春季学期学术系列讲座之十

题目：Global Analysis of Regulatory RNAs on Chromatin

讲座人：Xiang-Dong Fu, Ph.D.

Professor of Cellular and Molecular Medicine,

Department of Cellular and Molecular Medicine,

University of California, San Diego, CA

时间：2016年5月18日，13:00 - 14:30

地点：威尼斯wnsr666邓祐才报告厅

主持人：吴虹

摘要：

High eukaryotic genomes are populated with enhancers, but it has been a major challenge in defining enhancer-promoter relationships. Enhancers can also be divided into typical and super enhancers, yet their functional distinctions remain to be understood. To these and many others pressing questions of the 3D genome, we have developed a novel technology to detect Global RNA Interactions with DNA by deep sequencing (GRID-seq). By deducing general RNA background on chromatin, we unexpectedly detect a highly selective set of RNAs, including both long non-coding RNAs (lncRNAs) and protein-coding pre-mRNAs, that are decorated on enhancers, particularly super enhancers. Based on the origin of those RNAs and functional perturbation of enhancer activities, we are able to infer promoter-enhancer interactions that are significantly beyond the traditional framework.  These findings identify super enhancers as major hubs for genomic interactions and also suggest specific pre-mRNAs may have lincRNA-like function before processing into mRNAs.

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